카테고리 보관물: Fluorescence

Fluorescence

light source for Fluorescence

형광 관찰 조명장치의 종류

  • Mercury : 형광 현미경법에 가장 흔히 사용하는 광원으로, 자외선 및 가시광선 영역에서 아주 밝다.
Spectrum of mercury
  • Xenon : 가시광선에서 상대적으로 연속적인 스펙트럼을 가진다. 염료 및/또는 시료의 스펙트럼 특성을 정량적으로 분석하는 시스템에서는 제논 램프를 사용하는 것이 좋지만, 같은 와트수의 수은 램프만큼 밝지는 않다.
Spectrum of Xenon
  • LED :  약 10 ~ 40nm 범위의 상대적으로 좁은 스펙트럼 대역폭 내에서 빛을 방출한다는 고유의 특징이 있다. 일반적으로 정격 수명이 10,000시간 이상이고 50,000시간 이상 사용하는 경우도 많고, 전체 정격 수명에 걸쳐 출력 강도가 비교적 일정하게 유지된다.
Spectrum of LED

Laser : 레이저

  • 보통은 단색광의 좁은 스펙트럼 대역에서 극히 밝다. 하지만 레이저광의 간섭성으로 인해 샘플 영역에 균일한 조도로 비추기 어렵지만, Confocal 현미경에서는 갈바노 미러를 이용한 스캔 방식을 이용하여 이미지 획득시에 사용되어진다(LSCM).
    Spectrum of Laser

Metal-halide : 

  • 유효수명이 최대 2000시간으로, 일반적인 수은 램프의 200시간이나 제논 램프의 400시간과 비교하면 훨씬 길다.

Filter, Fluorescence, fw240624

JNO-G(L)

JNOPTIC Filter JNO-G(L)

– Filter set for TRITC / Cy3 Longpass
– Chroma Filter Model No. : 19004

JNO-G(L) Spectrum

Standard Green Excitaion filter (Long Pass type)
Standard Green Excitaion filter (Long Pass type)
FiltersType
AT540/25xEX
AT565DCBS
AT575lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(L)

TRITC VS JNO-G(L)
TRITC VS JNO-G(L)

JNO-G(L)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-G(L)
Filter Set : JNO-G(L) Camera : AcquCAM 23GR2 C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC
Fluorescence image taken using JNO-G(L)
Filter Set : JNO-G(L) Camera : AcquCAM 5G C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC
Fluorescence image taken using JNO-G(L)
Filter Set : JNO-G(L) Camera : AcquCAM 3G C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(L)필터에 적합한 형광색소

  • R-phycoerythrin

JNO-G(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Filter, Fluorescence, fw240624

JNO-B(L)

JNOPTIC Filter JNO-B(L)

– Filter set for GFP / FITC Longpass
– Chroma Filter Model No. : 19002

JNO-B(L) Spectrum

JNO-B(L) Spectrum
JNO-B(L) Spectrum
FiltersType
AT480/30xEX
 AT505DCBS
AT515lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

 * FITC VS JNO-B(L)

FITC VS JNO-B(L)
FITC VS JNO-B(L)

 * GFP VS JNO-B(L)

GFP VS JNO-B(L)
GFP VS JNO-B(L)

JNO-B(L)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-B(L)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : UPLNAPO40X
Fluorescence image taken using JNO-B(L)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 3G C-mount adapter : 1X Lens : UPLNAPO40X
Fluorescence image taken using JNO-B(L)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(L) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(L)필터에 적합한 형광색소

  • Acridine Orange + DNA and RNA
  • Auramine-rhodamine
  • JC-1 non-ratiometric

JNO-B(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. 02-3473-4188~9

CHROMA, Filter, Fluorescence, fw240624

JNO-U(L)

JNOPTIC Filter  JNO-U(L)

– Filter set for UV/DAPI Longpass
– Chroma Filter Model No. : 19000

JNO-U(L) Spectrum

JNO-U(L) Spectrum Chart
JNO-U(L) Spectrum Chart
FiltersType
AT375/28xEX
AT415DCBS
AT435lpEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(L)

DAPI VS JNO-U(L)
DAPI VS JNO-U(L)

JNO-U(L)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-U(L)
DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Lens : UPLNAPO40X, C-mount adapter : 1X Camera : AcquCAM 23GR2, Filter Set : JNO-U(L)
Fluorescence image taken using JNO-U(L)
DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Lens : UPLNAPO40X, C-mount adapter : 1X Camera : AcquCAM 3G, Filter Set : JNO-U(L)
Fluorescence image taken using JNO-U(L)
DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Lens : UPLNAPO40X, C-mount adapter : 1X Camera : AcquCAM 5G, Filter Set : JNO-U(L)

JNO-U(L)에 적합한 형광색소

  • Calcofluor White
  • DAPI

JNO-U(L) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Filter, Fluorescence, fw240624

JNO-R2(B)

JNOPTIC Filter  JNO-R2(B)

– Filter set for Cy5 / AlexaFluor 647 / Draq5
– Chroma Filter Model No. : 39007

JNO-R2(B) Spectrum

FiltersType
 AT620/50xEX
AT655DCBS
AT690/50mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* Cy5 VS JNO-R2(B)

Cy5 VS JNO-R2(B)
Cy5 VS JNO-R2(B)

 * AlexaFluor 647 VS JNO-R2(B)

AlexaFluor 647 VS JNO-R2(B)
AlexaFluor 647 VS JNO-R2(B)

 * Draq5 VS JNO-R2 (B)

Draq5 VS JNO-R2 (B)
Draq5 VS JNO-R2 (B)

JNO-R2(B)필터에 적합한 형광색소

  • Alexa Fluor 647™
  • Atto 647N
  • Cy5™
  • DiD
  • Draq5
  • DyLight 649
  • MitoTracker Deep Red 633/MeOH
  • Nile Blue
  • Quasar® 670
  • SYTO® 60
  • TO-PRO™-3

JNO-R2(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Filter, Fluorescence, fw240624

JNO-R1(B)

JNOPTIC Filter JNO-R1(B)

– Filter set for Texas Red / mCherry / AlexaFluor 594
– Chroma Filter Model No. : 39010

JNO-R1(B) Spectrum

JNO-R1(B) Spectrum
JNO-R1(B) Spectrum
FiltersType
AT560/40xEX
AT600DCBS
AT635/60mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* AlexaFluor 594 VS JNO-R1(B)

Alexa Fluor 594 VS JNO-R1(B)
Alexa Fluor 594 VS JNO-R1(B)

 * Texas Red VS JNO-R1(B)

Texas Red VS JNO-R1(B)
Texas Red VS JNO-R1(B)

 * mCherry VS JNO-R1(B)

mCherry VS JNO-R1(B)
mCherry VS JNO-R1(B)

JNO-R1(B)필터에 적합한 형광색소

  • Alexa Fluor 568™
  • Alexa Fluor 594™
  • CAL Fluor® Red 610
  • Cy3.5™
  • DyLight 594
  • FusionRed
  • Killer Red
  • mCherry
  • MitoTracker Red/MeOH
  • mKate2
  • mRFP1
  • ReAsH-CCPGCC
  • Rhodamine Red™-X
  • ROX
  • Sulforhodamine 101
  • Texas Red®
  • Texas Red®-X
  • X-rhod-1/Ca2+

JNO-R1(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Filter, Fluorescence, fw240624

JNO-G(B)

JNOPTIC Filter JNO-G(B)

– Filter set for TRITC / Cy3 / TagRFP / AlexaFluor546
– Chroma Filter Model No. : 39004

JNO-G(B) Spectrum

JNO-B(L) Spectrum
JNO-B(L) Spectrum
FiltersType
AT540/25xEX
AT565DCBS
AT605/55mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* TRITC VS JNO-G(B)

TRITC에 사용했을때 JNO-G(B)
TRITC에 사용했을때 JNO-G(B)

 * Cy3 VS JNO-G(B)

Cy3에 사용되는 JNO-G(B)의 스펙트럼 비교
Cy3에 사용되는 JNO-G(B)의 스펙트럼 비교

 * AlexaFluor546 VS JNO-G(B)

AF594에 사용되는 JNO-G(B)의 스펙트럼 비교
AF594에 사용되는 JNO-G(B)의 스펙트럼 비교

JNO-G(B)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-G(B)
Filter Set : JNO-G(B) Camera : AcquCAM 3G C-mount adapter : 1x Lens : UPLAPO20X Sample : bovine pulmonary artery endothelial cell, BPAEC
Fluorescence image taken using JNO-G(B)
Filter Set : JNO-G(B) Camera : AcquCAM 5G C-mount adapter : 1x Lens : UPLAPO40X Sample : bovine pulmonary artery endothelial cell, BPAEC
Fluorescence image taken using JNO-G(B)
Filter Set : JNO-G(B) Camera : AcquCAM 23GR2 C-mount adapter : 1x Lens : UPLAPO20X Sample : bovine pulmonary artery endothelial cell, BPAEC

JNO-G(B)필터에 적합한 형광색소

  • Alexa Fluor 546™
  • Alexa Fluor 555™
  • Atto 550
  • CAL Fluor® Red 590
  • Cy3™
  • DiI
  • DsRed
  • DyLight 549
  • MitoTracker Orange/MeOH
  • Resorufin
  • Rhod-2
  • TagRFP
  • TAMRA
  • tdTomato
  • Tetramethylrhodamine isothiocyanate
  • TRITC

JNO-G(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing.

Filter, Fluorescence, fw240624

JNO-B(B)

JNOPTIC Filter JNO-B(B)

– Filter set for EGFP / FITC / Cy2 / AlexaFluor488
– Chroma Filter Model No. : 39002

JNO-B(B) Spectrum

JNO-B(B) Spectrum Chart
JNO-B(B) Spectrum Chart
FiltersType
AT480/30xEX
 AT505DCBS
AT535/40mEM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* FITC VS JNO-B(B)

FITC VS JNO-B(B) spectrum chart
FITC VS JNO-B(B) spectrum chart

 * GFP VS JNO-B(B)

JNO-B(B) VS GFP spectrum chart
JNO-B(B) VS GFP spectrum chart

 * AlexaFluor488 VS JNO-B(B)

JNO-B(B) VS AlexaFluor 488
JNO-B(B) VS AlexaFluor 488

JNO-B(B)필터를 이용한 Sample Image 촬영

Fluorescence image taken using JNO-B(B)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 23GR2 C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence image taken using JNO-B(B)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 3G C-mount adapter : 1X Lens : PlanApo60xWLSM
Fluorescence image taken using JNO-B(B)
Sample : bovine pulmonary artery endothelial cell, BPAEC Filter Set : JNO-B(B) Camera : AcquCAM 5G C-mount adapter : 1X Lens : UPLNAPO40X

JNO-B(B)필터에 적합한 형광색소

  • Acridine Orange + DNA
  • Alexa Fluor 488™
  • Atto 488
  • Azami Green
  • BODIPY FL/pH7.2
  • Calcein
  • Calcium Green™-1
  • Cy2™
  • DiO
  • DyLight 488
  • EGFP
  • Emerald GFP
  • FAM
  • FITC
  • Fluo-4
  • GFP
  • LysoTracker Green/pH 5.2
  • MitoTracker Green FM/MeOH
  • mWasabi
  • Oregon Green™ 488
  • SYBR® Green I
  • SYTO 9/DNA
  • ZsGreen1

JNO-B(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than 26x38mm, please contact us for pricing. ( Tel. 02-3473-4188)

Filter, Fluorescence, fw240624

JNO-U(B)

– Filter set for DAPI / Hoechst / AlexaFluor350
– Chroma Filter Model No. : 39000

JNO-U(B) Spectrum

Spectra View for JNO-U(B)
Spectra View for JNO-U(B)
Filters Type
 AT375/28x EX
AT415DC BS
 AT460/50m EM

주요 형광 색소(Fluorochromes)와 스펙트럼 비교

* DAPI VS JNO-U(B)

DAPI VS JNO-U(B)
DAPI VS JNO-U(B)

* Hoechst VS JNO-U(B)

JNO-U(B) VS Hoechst
Spectrum Comparison by Wavelength JNO-U(B) vs Hoechst

 * AlexaFluor350 VS JNO-U(B)

JNO-U(B) vs AF350
Spectrum Comparison by Wavelength JNO-U(B) vs AF350

JNO-U(B)필터를 이용한 Sample Image 촬영

DAPI Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 23GR2, Filter Set : JNO-U(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X
DAPI
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X
DAPI, FITC, TRITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 23GR2, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X (OLYMPUS) Above three images are merged and processed using JNO-ARM
DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 23GR2, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X (OLYMPUS)
Above three images are merged and processed using JNO-ARM
DAPI, FITC, TRITC Sample : bovine pulmonary artery endothelial cell, BPAEC Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B) Objective lens : UPLNAPO40X, C-mount adapter : 1X Above three images are merged and processed using JNO-ARM
DAPI, FITC, TRITC
Sample : bovine pulmonary artery endothelial cell, BPAEC
Camera : AcquCAM 3G, Filter Set : JNO-U(B),B(B),G(B)
Objective lens : UPLNAPO40X, C-mount adapter : 1X
Above three images are merged and processed using JNO-ARM

JNO-U(B) 필터에 적합한 형광색소

  • Alexa Fluor 350™
  • Biosearch Blue
  • Coumarin
  • DAPI
  • DyLight 350
  • Hoechst 33258
  • LysoTracker Blue/MeOH

JNO-U(B) Spec

  • Typical Application(s) : Widefield Microscopy
  • Coating : Sputter/Hard Coated
  • Round excitation and emission filters mounted in anodized aluminum rings 2.3mm thick, up to 25mm in diameter (for standard sizes)
  • Rectangular dichroics up to 26x38mm, 1mm thick, to fit standard microscope manufacturer filter cubes (for standard sizes)

*** For filters larger than 25mm and dichroics larger than *** 26x38mm, please contact us for pricing.

Camera, Fluorescence, PHYSIOLOGY

AcquCAM 23S

AcquCAM 23S

(Down load Catalog ACQUCAM 23S)

  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O

AcquCAM 23S
EXMOR Sensors: Next-generation imaging sensors based on CMOS, increase the strength and reduce noise.

 

High sensitivity camera available from visible to IR range

20151120_1(CA1 pyramidal neuron)
Apply high sensitivity EXMOR sensor with additional filter block visible range, IR Cut filter image could be observed.

 

Available wider range of observation

AcquCAM 23S_6
By applying a 1 / 1.2 ” EXMOR sensor, you can observe a wider range of superior sensitivity image.
These images were shot by AcquCAM 23S Package.

High sensitivity and fast frame image

AcquCAM 23S_(CA3 pyramidal neuron)

AcquCAM 23S_(Dentate gyrus Granule cell)
To maximize the range of imaging possible observation with fast frame 54Fps and high sensitivity 0.015 lx .

[Specification]

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats

& Frame rate

 1920×1200 Y16 @ 25 fps

1920×1200 Y800 @ 54 fps
Sensitivity 0.015 lx
Exposure time 1/100000 to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65 g