Fluorescence In Situ Hybridization
– 형광동소보합법 –
Inadequat Contrast Image 
Good Contrast Image 
Inadequat Contrast Spectrum Chart 
Good Contrast Spectrum Chart
Many factors influence the quality of FISH
| 1. Processing & storage | 2. Specimen | 3. Probes |
| 4. Microscope | 5. Filters | 6. Light source |
| 필터 선정시 고려 할 사항 | 설 명 |
|---|---|
| Contrast | Signal / Noise ratio = MORE CONTRAST |
| Brightness | Save time, increase throughput and reduce eye strain with brighter images |
| Color Separation | Distinguish between colors more easily and exclude unwanted colors |
| Registration | Each filter set produces images that are aligned with images from other filter sets |