J.H.JIN의 모든 글

AcquCAM 3

2024년 1월 10일 J.H.JIN

TOMORO AcquCAM 3

TOMORO ACQUCAM 3는 현미경 영상 획득을 위해 설계되었습니다. 3백만 화소의 이미지 소자는 현미경 영상을 대한 최적화 된 솔루션을 제공합니다. USB2.0 인터페이스를 통하여 하나의 케이블 연결만으로 300만화소의 이미지를 초당 10 프레임으로 관찰 할 수 있으며 최대 35.5 프래임의 고속으로도 현미경 영상을 관찰할 수 있습니다.

Features

3 Mega pixel Micron MT9T031 CMOS Sensor
Color, Progressive scan
High Speed USB 2.0 Interface (480Mbits/sec)
10 fps at 2560×1536 resolution / up to 35.5 fps at 1024×768 resolution
Auto White Balance support
RGB Bayer video output
Hot pluggable
All camera parameters adjustable via the computer
Preview Software provided (TOMORO Capture)
Software compatible with Windows 2K/XP and Vista (32/64bit) operating systems
Fully integrated with OLYMPUS ‘analySIS’ image analysis softare not TWAIN

Specifications

Sensor	Micron MT9T031
Type	CMOS Progressive scan
Size	1/2 inch
Resolution	H:2560, V:1536
Pixel size	H:3.2um, V:3.2um
Video formats &(Frame rates)	10 Fps at Full resolution
Video formats &(Frame rates)	Up to 35.5 Fps at XGA (1024×768) resolution
Sensitivity	5 lx at 1/30s, gain 20 dB
Exposure time	100us to 2sec
White balance	Auto / Manual
Lens mount	C/Cs mount
Interface	Mini USB 2.0 interface 480Mbit/s
Power supply	4.5 to 5.5 VDC via USB 2.0
Dimensions	H:50.6mm, W:50.6mm, L:29mm
Mass	Approx 140g

Setup Driver & Software Download

AcquCAM, Camera

AcquCAM Specification Comparison Table

2024년 1월 9일 J.H.JIN

일반 현미경용 카메라

MODEL No.	BKONV KON3.1	AcquCAM 3	AcquCAM 5/U3
Connect	USB 2.0	USB 2.0	USB 3.0
Sensor	CMOS	CMOS	CMOS
Chip Size	1/2 inch	1/2 inch	1/2.5 inch
Resolution	2048×1536	2048×1536	2592×1944
FPS(Max)	8	10	15
SHUTER		rolling	rolling
Trigger	x	x	o
Shutter	128㎲ ~ 2s	100㎲ ~ 4s	50㎲ ~ 0.25s
IR Cut	650㎚ ~	650㎚ ~	650㎚ ~
Sensitivity	–	–	0.05 ㏓
Pixel Size (µm)	H: 3.2㎛, V: 3.2㎛	H: 3.2㎛, V: 3.2㎛	H:2.2㎛, V:2.2㎛

형광 관찰이 가능한 고감도 카메라

MODEL No.	AcquCAM Pro/G3	AcquCAM 3G	AcquCAM 5G	AcquCAM 23GR2
Connect	USB 3.0	USB 3.0	USB 3.0	USB 3.0
Sensor	CCD	Pregius	Pregius	Pregius
Chip Size	1/3 inch	1/1.8 inch	2/3 inch	1/1.2 inch
Resolution	1280×960	2048×1536	2448×2048	1920×1200
FPS(Max)	30	60	35	162
SHUTER	global	global	global	global
Trigger	o	o	o	o
Shutter	100㎲~ 30s	20 ㎲ ~ 30s	25 ㎲ ~ 4s	20㎲ ~ 30s
IR Cut	650㎚ ~	650㎚ ~	650㎚ ~	Option: 700㎚~
Sensitivity	0.05 ㏓	0.05 ㏓	0.05 ㏓	0.05 ㏓
Pixel Size (µm)	H:3.75㎛, V:3.75㎛	H:3.45㎛, V:3.45㎛	H:3.45㎛, V:3.45㎛	H:5.86㎛, V:5.86㎛

전기 생리학 실험 전용 카메라

MODEL No.	AcquCAM Silver	AcquCAM Pro/S3	AcquCAM 23S2
Connect	USB 2.0	USB 3.0	USB 3.0
TYPE	Mono	Mono	Mono
Sensor	CCD	CCD	Pregius
Chip Size	1/1.8 inch	1/3 inch	1/1.2 inch
Resolution	1600×1200	1280×960	1920×1200
FPS(Max)	12	30	162
SHUTER	global	global	global
Trigger	x	o	o
Shutter	100㎲ ~ 30s	100㎲ ~ 30s	20㎲ to 30s
IR Cut	Option: 700㎚ ~	Option: 700㎚ ~	Option: 700㎚ ~
Sensitivity	0.05 ㏓	0.015 ㏓	0.015 ㏓
Pixel Size (µm)	H:4.4㎛, V:4.4㎛	H:3.75㎛, V:3.75㎛	H:5.86㎛, V:5.86㎛

Setup Driver & Software Download

Image Pro

Image-Pro v11

2024년 1월 4일 J.H.JIN

ImagePro11 Feature Introduction

[100-102] Image-Pro v11
2D Analysis Bundle

[100-001]
Image-Pro – Base

File Management, 2D Viewer, Process/Adjust, Usability, Data Management, Reports, Auditing, Scripting

[300-011]
2D Measurements – Module

Direct & Assisted Measurements, Auto Shape & Edge Detection, Classification, & Color Measurement.

[300-012]
2D Automated Analysis – Module

Smart Segmentation, Classification, 2D Tracking, & the Essentials Protocol Collection with 3 Protocols.

Object Count	Percent Area
Objects within Objects

Includes 3 Protocols
상기 테이블의 기능은 하기의 동영상에서 확인하실 수 있습니다.

[Option] Image-Pro 11
Protocols

[300-013]
Protocols – Cell Biology – Extension

Apoptosis	Autophagy
Cell Count	Cell Morphology
Cell Proliferation	Confluence
Lipid Droplet	Live/Dead
Ring	Transfection 1 Label Transfection 2 Label

Includes 10 Protocols
상기 테이블의 기능은 하기의 동영상에서 확인하실 수 있습니다.

Apoptosis

Autophagy

Cell Count

Cell Morphology

Cell Proliferation

Confluence

Lipid Droplet

Live/Dead

Ring

Transfection 1 Label
Transfection 2 Label

[300-014]
Protocols – Cell Biology Plus – Extension

Angiogenesis	Colocalization
Neurite Outgrowth	Translocation Total Cellular Area
Wound Healing	Translocation Area Above Threshold

Includes 5 Protocols
상기 테이블의 기능은 하기의 동영상에서 확인하실 수 있습니다.

Angiogenesis

Colocalization

Neurite Outgrowth

Translocation Total Cellular Area

Wound Healing

Translocation Area Above Threshold

[300-015]
Protocols – Materials – Extension

Composite Materials	Layers Concentric
Layers Curved	Layers Parallel
Fiber Separation	Fiber Thickness
Particles Size	Particles Phase
Phase	Pores

Includes 10 Protocols
상기 테이블의 기능은 하기의 동영상에서 확인하실 수 있습니다.

Composite Materials

Layers Concentric

Layers Curved

Layers Parallel

Fiber Separation

Fiber Thickness

Particles Size

Particles Phase

Phase

Pores

Features added to Image-Pro v.11
(Compared to Image-Pro v.10)

Image Analysis

ImagePro11 Compared to ImagePro10

2023년 11월 23일 J.H.JIN

Features added to Image-Pro v.11
(Compared to Image-Pro v.10)

Image Management & Display

Image & MetaData Management

Channel Management – Save unique characteristics per channel

Views & Overlays

Well Plate Viewer
Color-Coded Well Plate Heat Maps

Processing & Adjustments

Search & Help

Universal Search across Recently Used items & Help topics
Dockable & Dynamic Help panel for easy access to Documentation

Licensing

Flexible License Linking/Unlinking to Computer or License Server in addition to USB dongles
Online License “Bundle” management – Control module configuration per base product

Data Analytics & Reporting

Data Collection & Display

Display Data using Heat Maps
Display annotated images in Data Table along with statistical data

Auditing & Authentication

Scripting & Automation

2D Measurements

Classification

Adjust measurement overlay appearances by class

2D Automated Analysis

Protocol-based Segmentation

Run on single file multi-positional (including multi-well) datasets or collections of individual images
Bi-direction feedback between data tables & annotated images & charts
Library of Protocol reports for both large datasets & single images
Analysis Protocols – Essentials Collection (Objects within Objects, Object Count, % Area)
Analysis Protocols – Cell Biology Collection (Apoptosis, Autophagy, Cell Count, Cell Morphology & more)
Analysis Protocols – Cell Biology Advanced Collection (Angiogenesis, Colocalization, Neurite Outgrowth, & more)
Analysis Protocols – Materials Collection (Particles, Pores, Particle Phase, Fiber Thickness, & more)

2D Image Capture

Multi-Channel Capture

LightSource Device Control
Acquisition of mixed multi-channel & transmitted light datasets
Automated capture of multichannel image-set (w/ a supported multi-channel light source)
Guided acquisition of multi-channel image-sets from a manual microscope

Real-Time Deconvolution

2D Real-Time Deconvolution integrated with Capture
Wiener Filter-based algorithm
2D Iterative-based algorithm

3D Visualization & Analysis

3D Visualization

3D viewer with advanced control & volume rendering (Min & Max Projections)
Clipping plane viewing
Slicer viewing

3D Visualization of Big Data Sets

Convert Large Image Sets Into Pyramidal Tiff for Big Data Visualization

AutoQuant Deconvolution (Post-Acquisition)

2D Fixed & Adaptive Deconvolution Algorithms
Support of both Theoretical & Measured PSFs
GPU Acceleration
PSF generation for WF, CF, 2 Photon, Spinning Disk, & STED modalities
Spherical Abberation Correction
Batch Deconvolution

License Server
(for Network-based Licensing)

Bundles can be left as floating licenses or can be fixed to a single workstation
Bundle assignment to License Server can be adjusted anytime as needed
User time out returns license back to License Server

Image-Pro v.11
(Video Materials for Protocol Introduction)

260529, JNOPTIC

JNO-ICG

2023년 6월 8일 J.H.JIN

Indocyanine Green (ICG) 관찰을 위한 실체 현미경

Stereo-microscope for Indocyanine Green

Images captured with JNO-ICG

Indocyanine Green (ICG) _ Bright Field Image — Indocyanine Green (ICG)
Bright Field Image
Imaging Device : AcquCAM 23GR2

Indocyanine Green (ICG) _ Fluorescent Image — Indocyanine Green (ICG)
Bright Field Image
Imaging Device : AcquCAM 23GR2

Indocyanine Green (ICG) _ Fluorescent & Bright Field Image — *Indocyanine Green (ICG)*
*Fluorescent & Bright Field Image*

JNO-ICG에 관련한 문의는 하기의 담당자에게 연락바랍니다.

개발자( 진재환 ) _ 광학현미경 관련 이력

E-mail : jhjin@jnoptic.com 
Mobile : + 82-2-3473-4188~9

Light Source

CoolLED Specrtrum

2022년 12월 19일 J.H.JIN

Light source for Microscopy

CHROMA, Fluorochrome

Fluorochrome – Cy7™

2022년 4월 13일 J.H.JIN

Cy7™

Spectrum Chart

Excitation (㎚)	Emission (㎚)
743	767

Recommended Filter set

일반 형광 Lighting source

Model No.	Excitation	Dichroic mirror	Emission	Remarks
JNO-49007	ET710/75x	T760lpxr	ET810/90m	–
JNO-49037	ET740/40x	T770lpxr	ET780lp	–

Cy7 & JNO-49007 Specrtum Chart for CoollED pE4000

Cy7 & JNO-49037 Specrtum Chart for CoollED pE4000

Cy7을 관찰하기 위한 형광 광원 장치
- T. 02-3473-4188로 연락 주시기 바랍니다.

Information

시아닌은 여전히 업계에서, 그리고 최근에는 생명 공학 (라벨링, 분석)에서 사용되고 있습니다. 시아닌은 형광 염료로서, 특히 생체 의학 이미징에서 많은 용도로 사용되며 구조에 따라 IR에서 UV까지 스펙트럼을 커버합니다.

Cyanine은 polymethine 그룹에 속하는 합성 염료 계열의 비 계통적 이름입니다. cyanin이라는 단어는 일반적으로 청록색 ( “아쿠아”에 가까운)의 그늘을 의미하는 영어 단어 “cyan”에서 유래되었으며 다소 다른 색상 “진한 파란색 “을 의미하는 그리스어 κυάνεος / κυανοῦς kyaneos / kyanous에서 파생되었습니다.

Cy7은 육안으로는 보이지 않는 근적외선 형광체이며, Cy7.5 염료와 함께 생체 내 이미징 어플리케이션에 사용됩니다.

Cyanines (Cy)는 Fluorescein (FITC) 및 rhodamines (TRITC, RRX)와 같은 기존의 염료를 대체하여 보다 밝고 안정한 형광을 생성합니다.

Cyanines were and are still used in industry, and more recently in biotechnology (labeling, analysis). Cyanines have many uses as fluorescent dyes, particularly in biomedical imaging. Depending on the structure, they cover the spectrum from IR to UV.

Cyanine is the non-systematic name of a synthetic dye family belonging to polymethine group. The word cyanin is from the English word “cyan”, which conventionally means a shade of blue-green (close to “aqua”) and is derived from the Greek κυάνεος/κυανοῦς kyaneos/kyanous which means a somewhat different color: “dark blue”.

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 ㎚). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Cyanines(Cy) replace advantageously conventional dyes such as Fluorescein(FITC) and rhodamines (TRITC, RRX), yielding brighter and more stable fluorescence.

Fluorescence